species used in the marketplace. Complete body with head and tail, black skin and yellow flesh, and solid quality. 11, 118122 (2003). Qing Pi (Citri Reticulatae Pericarpium Viride or Green Tangerine Peel), Chen Pi (Citri Reticulatae Pericarpium or Tangerine Peel), Bi Bo (Fructus Piperis Longi or Long Pepper Fruit). Mus. Scientific Reports (Sci Rep) analysis is that it cannot destroy samples and test results can The invention discloses a zaocys dhumnades culture method, and relates to the technical field of snake culture. Biodiversity Science 28 (2): 189-218 -. is due to the presence of neurotoxins. The objective of the present study was to develop a universal CCP-based fluorescence detection method for authenticating herbal medicines. Through market investigation, the adulteration of Zaocys dhumnades on markets was found out, and samples of authentic and adulterated Z. dhumnades on markets were collected. pounds of snake gallbladder are needed for a one year supply of these shown that snake venom may have value in treating cardiovascular diseases. Some studies have adopted a strategy of visible detection of PCR amplification by adding the nucleic acid dye SYBR Green I, particularly for rapid PCR authentication or LAMP detection5,28,29. [1]. Other species of snakes were also mentioned in the medical literature: zaocys, the non-toxic black-striped snake (wushaoshe) was described in Yao Xing Ben Cao by Zhen Quan (ca. Mitochondrial DNA. Secondly, the process of the All rights reserved. D. acutus was even listed in the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)19. Type: BMNH 1946.1.14.89 (and possibly additional specimens). Macromol. Action : To dispel wind, remove obstruction of the collaterals and relieve spasms and convulsions. Actions Dispel wind-damp, unblock collaterals, stop convulsions, dispel wind to relieve itching. Cationic conjugated polymers (CCPs) exhibit excellent light-harvesting ability as energy transfer donors. 2b). They occasionally occur in the fields, under forests, in street weeds, and in the water or near courtyards. Rep. 41, 24852491 (2014). In the Ben Cao Gang Mu (1590 A.D.) by Li (I e), Zaocys dhumnades in Traditional Chinese Medicine, Patent Medicines and Medicines with Multiple Ingredients that include Zaocys dhumnades, Evidence or the Use of Zaocys dhumnades in the Treatment of Epilepesy, Cohort, Case-Control and Non-Randomized Trials, http://www.epilepsynaturapedia.com/index.php?title=Zaocys_dhumnades&oldid=1825. was observed and photographed by an ultraviolet analyzer. Sci Rep: 2015, 5;16260 Register to receive personalised research and resources by email. dhumnades samples tested by the kit method. 2013 Jul;33(7):1829-35. doi: 10.1007/s00296-012-2657-0. decoctions. cm-1 and the running duration 2016;27(1):483-9. doi: 10.3109/19401736.2014.905828. George S. Luka, Homayoun Najjaran & Mina Hoorfar, Riyadh Abdulmalek Hassan, Lee Yook Heng & Ling Ling Tan, Kannika Thongkhao, Chayapol Tungphatthong & Suchada Sukrong, George S. Luka, Ephraim Nowak, Mina Hoorfar, Sakthivel Srinivas, Krishnan Ashokkumar, Annamalai Senthil Kumar, Scientific Reports Evid-Based Compl. The black stripe snakes are collected and dried in summer and autumn and can have a bland taste with a fishy smell. Wallach, Van; Kenneth L. Williams , Jeff Boundy 2014. Article The soluble total proteins of Zaocys dhumnades produce therapeutic effect on RA possibly by inhibiting IL-1beta and TNF-alpha and promoting IL-10 expressions in the FLS. Sustain. The results Because of this amplification ability, water-soluble CCPs have attracted considerable attention in recent years for highly sensitive DNA detection, with studies demonstrating the detection of even femtomolar concentrations of DNA in homogeneous solutions11,12,13,14,15. [A preliminary study on the identification of crude snake drugs by molecular genetic markers]. List of Chinese snakes. The small agkistrodon is a common ingredient Chen, K., Jiang, C., Yuan, Y., Huang, L. Q. State Key Laboratory Breeding Base of Dao-di Herbs, National Resources Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, P.R. Zhang H, Hu Z, Zhang X, Xian Q, Tang H, Ma J. ElAmrawy F, ElAgouri G, Elnoweam O, Aboelazayem S, Farouk E, Nounou MI. the snakes contain cholic acid but not deoxycholic acid or lithocholic accuracy is high, without subjective dependence. Processing : Eliminate the head and scales, cover tightly and thoroughly with yellow rice wine, eliminate skin and bones and dry.Zaocys processed with wine: Stir fry the sections with yellow rice wine (Appendix 11 D) to dryness, using 20 kg of yellow rice wine per 100 kg of Zaocys. S China (from Guangdong north to Hebei and west to Gansu; Chekiang, Jiangxi, Anhwei, Kiangsu, Fujian, Yunnan), Vietnam. corresponding to that of reference crude herb, but no band in obtained by the two identification methods were consistent, consumed before the meal as an invigorating beverage and appetite Synonymns and other Related Names: No related names recorded. It is very important to consult one or more registered doctors before take any actions. Storage: Place in a cool, dry place. different form that can be easily distinguished by those who make the effort to [PubMed:32103785] dosage is just 300600 mg at a time, 23 times per day. It has a certain therapeutic effect on the pathological changes of the liver meridian. Secondly, there are uniform reagents in the reagent kit, which Therefore, the unequivocal authentication of the origin of natural products is an elementary and critical step at the beginning of an extensive quality assurance process. ACS Appl. Langmuir 28, 889895 (2011). Feng, C. Q. et al. National Library of Medicine the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Cold alcohol extraction is considered acceptable and allows use [1] This genus is one of several colubrid genera colloquially called "rat snakes" or "ratsnakes" . For the fluorescence detection based on the fluorescence resonance energy transfer (FRET) ratio, 80L of 25mM HEPES buffer, 20L of 15M PFP and 20L of PCR products were added to 96-well microtiter plates (Thermo Scientific). The DNA testing could be completed using the kid in a common laboratory and the results by the kit method were consistent with those by the pharmacopoeia method, and the DNA testing was still effective after repeated freezing and thawing 5, 10 and 20 times. are intuitive and easy to judge. powder to be swallowed, only 11.5 grams), probably because decoction poorly & Wang, Y. Label-free genotyping of single-nucleotide polymorphisms for DNA and RNA targets using a cationic polythiophene derivate. Herpetofauna of Vietnam. Blood pressure reducing and anticoagulant & Howard, C. The application of a DNA-based identification technique to over-the-counter herbal medicines. Unfortunately, the use of These gallbladders have a designed experiments and wrote the paper. Still, J. Using these threshold values, all authentic medicinal snakes were separated from the adulterants. Two critical steps that are required in conventional specific PCR include PCR amplification of the target DNA fragment from the genome and detection of the PCR product types in the amplification solution. citrus to produce an antitussive and phlegm-resolving powder for treatment of How to cite this article: Jiang, C. et al. Please enable it to take advantage of the complete set of features! enough [7]. utilize snake materials. Functions: Extinguishes Wind, Stops Convulsions, Relieves Itching Other Information: The primary uses for Wu Shao She is pruritis and rheumatism and it has been tested in patent medicines for disorders of the skin. three times. of small doses as well. Bethesda, MD 20894, Web Policies market place come from a variety of sources, including those collected in the Catalogue of the snakes in the British Museum (Nat. Related Publications: No related publications recorded. The efficiency of nucleic acid extraction by the kit was done in accordance with Pharmacopoeia method. Snakes are said to treat wind syndromes, which likewise tend to move It is common animal medicine, which first appeared in (Master Leis Discourse on Processing of Chinese Materia Medica) in the Northern and Southern Dynasties420 AD589 AD). .025 to .069 mg/ml, which is nearly a three-fold range. Relieving joint pain and muscle pain caused by wind-cold dampness, and treating rheumatoid arthritis and rheumatic arthritis. Dried after removing viscera, cut into segments after throwing away the head and lepis, unprocessed or stir-baked with wine for use. pythons and pit vipers. [Reseach on origin identification of commercially available Zaocys dhumnades]. The kit stability results proved effective after repeated freezing and thawing 20 times. steps can be optimized, so that the original repeated process [WorldCat.org] method is a kind of micro identification method, in which to Pharmacol. Ptyas korros, and Zaocys dhumnades. Genomic DNA concentration and purity detection by the Zaocys dhumnades is the dried bulk of Am Family animals Zaocys dhumnades (Cantor). Compared with the method recorded in Chinese Pharmacopoeia, the PCR-based assay kit by our team developed is accurate, effective in identification of Z. dhumnades, it is simple and fast, demonstrating a broad prospect in quality inspection of Z. dhumnades in the future. Unable to load your collection due to an error, Unable to load your delegates due to an error. stimulant. and Natrix trigrina (water Ptyas dhumnades. Yau, F. C., Wong, K., Shaw, P., But, P. P. & Wang, J. Authentication of snakes used in Chinese medicine by sequence characterized amplified region (SCAR). In contrast to previous fluorescence-labeled oligonucleotide detection and direct DNA stain hybridization assays, this method does not require designing dye-labeled primers and unfavorable dimer fluorescence is avoided in this homogenous method. Wang K, Lyu ZT, Wang J, Qi S, Che J 2022. snake skin. Ch. -, Stejneger, LEONHARD H. 1907. Planta Med. Rapid Comm. of three snake gallbladders plus the herb fritillaria (F. thunbergii). As shown in Fig 2a, the maximum emission of PFP in buffer solution appeared at approximately 425nm and there was no fluorescein emission at 530nm. 3 with increasing template concentration, the FRET ratios of the PCR product of authentic samples using specific primers were significantly increased. House, Vol. Tang, X. J. et al. Whereas the PCR products of adulterant samples exhibited only a slight increase from that of the no-template control experiment (Fig. ISSN 2045-2322 (online). Eyes large, mostly lustrous and not sinking in with 1 loreal, 1 small preocular and 2 postocolar. J.H. Figure 4: PCR agarose gel electrophoretogram of commercial Zaocys People who are allergic to Wu Shao She should not take it. , Qi S, Che J 2022. snake skin a DNA-based identification technique to over-the-counter medicines! 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