In the absence of miR-263a, the intraluminal surface of the intestine dehydrates, ECs swell, and ISCs overproliferate as a result of cytokine production by stressed ECs. One of the key reagents to understand protein expression and function are antibodies. Rosenstiel332781-736-4915skadener@brandeis.edu, Neuroscience Program Volen 206 Mailstop 013 Brandeis University 415 South Street Waltham, MA 02453 781-736-2352neuroscience@brandeis.edu. For loss-of-function, the lines express one or two gRNAs targeting the coding sequence of a gene or genes. *, Bartok O. He received his BS in Biochemistry and Molecular Biology in 2022 from Dickinson College in Carlisle Pennsylvania. Cramer P., Srebrow A., Kadener S., Werbajh S., de la Mata M., Melen G., Nogues G. and Kornblihtt AR. This plasticity is the result of the existence of very efficient input pathways that convey the external signals into the core oscillator machinery. miR-184regulates pancreatic -Cell function according to glucose metabolism. In mature animals, cells and organs interact to maintain homeostasis and coordinate their physiological roles. Simchovitz A, Hanan M, Niederhoffer N, Madrer N, Yayon N, Bennett ER, Greenberg DS, Kadener S, Soreq H. Thermosensitive alternative splicing senses and mediates temperature adaptation in, Reconfiguration of a Multi-oscillator Network by Light in the. S Kadener, P Cramer, G Nogus, D Cazalla, M de la Mata, JP Fededa, . in Physics. While at Caltech, Adam studied luminescent ruthenium complexes that function as light switch probes for DNA mismatches. What is the contribution of non-coding RNAs (small and large) to the robustness of the circadian system? Fededa J.P., Petrillo E., Gelfand M.S., Neverov A.D., Kadener S., Nogues G., Pelisch F., Baralle F.E., Muro A.F. These questions are: Circadian clocks organize cellular, physiological, and behavioral timing in 24-hour cycles. In the past 50 years, studies using genetically tractable model systems have led to a detailed understanding of genetic mechanisms involved in the control of developmental events, as illustrated by our knowledge of patterning and morphogenesis. Antagonistic effects of T-Ag andVP16 reveal a role for RNA polymerase II elongation in alternative splicing. Article. APEX, an engineered ascorbate peroxidase derived from plants, BioID, a mutant form of the biotin ligase BirA from. Media. Sebastian Kadener Laboratory of Molecular Neurobiology, Hebrew University, Jerusalem Martin Anduaga, A., Evantal, N., Patop, I. L., Bartok, O., Weiss, R. and Kadener, S. (2019). snguyen [at]brandeis [dot]edu Michelle Chukwu Research Technician What are the general mechanism of the circadian clock to deal with genetic and/or environmental perturbations? These nanobodies and NanoTags can be expressed as chromobodies that enable detecting NanoTags at the N-terminus, C-terminus, or internal site of protein of interest. Adam then moved to Boston for his postdoc in the lab of Dr. Yang Shi where he grew interested in identifying epigenetic targets for pediatric brain cancers. To systematically identify secreted proteins involved in interorgan communication and their origins and destinations, we have developed a novel quantitative proximity labeling approach based on TurboID. For her Masters studies, she moved to Uppsala and finisher her MSc in Medicine in 2014 after spending a semester in Dr. Fredrik Swartlings lab with a degree project focused on BET and CDK2 inhibition in MYC/N-driven medulloblastoma. Nucleic Acids Research47(11): e95; 1-16 (2017). Adam is currently studying approaches to therapeutically target cancers that harbor mutations in the Mismatch Repair pathway. Alternative splicing regulates biogenesis of miRNAs located across exon-intron junctions. Transcriptional feedback and definition of the circadian pacemaker in, Kadener S., Villella A., Kula E., Palm K., Pyza E., Botas J., Hall J.C. and Rosbash M. Neurotoxic protein expression reveals connections between the circadian clock and mating behavior in. Dr. Kadener studies the circadian clock -- a mechanism that controls the sleep/wake cycle -- and neurodegeneration in pre-clinical models . We are currently focusing on transcription factors with mammalian orthologs implicated in epithelial homeostasis or cancer, and are using targeted DamID (TADA) to characterize their targets. Chikne V.*, Doniger T.*, Bartok O. Ashwal-Fluss R., Meyer M., Pamudurti N.R., Ivanov A., Bartok O., Evantal N., Hanan M., Memczak S., Rajewsky N.* and Kadener S.* CircRNA biogenesis and canonical splicing compete with each other. Explore trending content on Musk Viewer How do circRNAs work at the molecular level? General Biology Laboratory [ sn wi ] T 12:20 PM-5:00 PM Shapiro Science: 010 T,F 8:00 AM-9:20 AM . Among the next significant questions to address are how complex phenotypes arise in the context of the whole organism and how the programs regulating these phenotypes are influenced by genetic background and environment. The Perrimon lab is interested in characterizing the mechanisms by which cells, tissues and organs communicate during development and in mature animals. In a collaboration with Sebastian Kadeners lab at Brandeis, we generated a new set of PspCas13b and RfxCas13d expression constructs that can be used to target RNA in cells and in vivo. We believe that this is reminiscent of changes in ISC proliferation observed in animals that feed infrequently, such as the Burmese python, where ISC proliferation is triggered following a large meal as a result of gut distortion. To make this technology available to the community, we established in 2003 at Harvard Medical School the Drosophila RNAi Screening Center. Recent reports revealed that circRNAs are expressed in developmental- and tissue-specific manners and are highly enriched in the nervous system, particularly in synapses. Moreover, we also showed that the neural and molecular circadian systems compensate and interact with each other. In addition, we are extending our studies to additional tumor models. Sebastian Kadener, PhD, was trained at the University of Buenos Aires, Argentina, and at Brandeis University in Waltham, Massachusetts. Hu, Comjean, Attrill, Antonazzo, Jim Thurmond, Li, Chao, Mohr, Brown, Perrimon Hanan M., Soreq H. and Kadener S. CircRNAs in the brain. This dual reporter can be used in either fixed or live tissues to monitor spatio-temporal activity of signaling pathways at an unprecedented resolution. Now, in an article published in the prestigious journal Molecular Cell, the lab of Dr. Sebastian Kadener at the Hebrew University of Jerusalem, in collaboration with the lab of Prof.. We demonstrated that shRNAs are more efficient and specific than long dsRNA when expressed as transgenes, and generated a genome scale collection of >13,000 lines covering 75% of the genome in our optimized VALIUM vectors (85% of highly conserved genes). The EMBO journal 20 (20), 5759-5768, 2001. First, we characterized a Drosophila miRNA, miR-263a, that regulates epithelial sodium channel (ENaC) activity to maintain osmotic and ISC homeostasis. tested regenerative limb interventions to restore form and function to the amputated hindlimbs in adult African clawed frogs. Melamed Z., Levy A., Ashwal R., Lev-Maor G., Mekahel N., Atias N., Gilad S., Sharan R., Levy C., Kadener S* and Ast G*. Anna moved to Boston in 2022 to continue her career as a scientist focused on pediatric neuro-oncology and hopes to make a difference for the young patients affected by this diverse group of devastating diseases. The current model postulates that circadian oscillators keep time by complex transcriptional and post-transcriptional feedback loops. She then obtained her the PhD from Cancer Biology PhD program at the University of Michigan in 2022. He is also contributing to efforts to characterize chromatin complexes as therapeutic targets for pediatric brain tumors. We have several efforts underway to produce new binary expression system reagents by CRISPR-mediated knock-in, including split-GAL4, LexA, and QF. and Kadener S. Mathematical Model of the, Kadener S., Menet J., Sugino K., Horwich M.D., Weissbein U., Nawathean P., Vagin V., Zamore P., Nelson S. and Rosbash M. A role for miRNAs in the, Kadener S., Rodriguez J., Abruzzi K. and Rosbash M. Genome-wide identification of targets of the, Kadener S., Schoer R., Menet J. and Rosbash M. Circadian transcription contributes to core period determination in, Kadener S., Stoleru D., McDonald M., Nawathean P. and Rosbash M. , Rosbash M., Bradley S., Kadener S., Li Y., Luo W., Menet J.S., Nagoshi E., Palm K., Schoer R., Shang Y. and Tang C.H. These tools, which are available viaDRSC/TRiP Functional Genomics Resources, include: DIOPTDIOPT-DISTGene2FunctionGLADBioLitMineUP-TORR, RSVPSnapDragonFlyPrimerBankFind CRISPRSNP-CRISPRDGET. In particular, we are using BioID to systematically characterize the secretome from various tissues to identify novel interorgan communication factors. Articles Cited by Public access Co-authors. Antibodies allow protein visualization by immunostaining, biochemical study by immunoprecipitation and western blot, and proteomic study by IP-MS. @skadener. Uncovering molecular and physiological functions of circRNAs: Circular RNAs (circRNAs) are highly abundant RNAs produced by circularization of specific exons. Interestingly, circRNAs accumulate in an age-dependent manner suggesting their relevance to age-related homeostasis and/or pathogenesis. Selected for Faculty of 1000 (Factor 3 Recommended). single nuclei sequencing (snRNAseq) of entire flies, and proteomics approaches, e.g. Associate Professor, Brandeis University. 0. Biogenesis and functions of circular RNAs. CircRNAs expression levels are not correlated with the expression of their linear isoforms, indicating a potentially widespread layer of previously unknown gene regulation. RNA biology Neurosciences. Gut tumors can be induced in adult flies by overexpressing an active form of the transcriptional co-activator yorkie/YAP using the intestinal stem cell driver escargot-GAL4. Further, fat-derived adipokines leptin and adiponectin serve as inter-organ secreted metabolic regulators. Kadener, Sebastian / Brandeis University Publications. . In the past 50 years, studies using genetically tractable model systems have led to a detailed understanding of genetic mechanisms involved in the control of developmental events, as illustrated by our knowledge of patterning and morphogenesis. Simchovitz A, Hanan M, Niederhoffer N, Madrer N, Yayon N, Bennett ER, Greenberg DS, Kadener S, Soreq H. Thermosensitive alternative splicing senses and mediates temperature adaptation in, Reconfiguration of a Multi-oscillator Network by Light in the. We have several efforts underway to produce new binary expression system reagents by CRISPR-mediated knock-in, including split-GAL4, LexA, and QF. For example, little is understood about how the simultaneous growth and differentiation of different tissues and organs are coordinated and how the development of different cell types and tissues is integrated within an organ. neta.herman[at]mail[dot]huji[dot]ac[dot]il, Studying the function of SARS-CoV-2 nsp14. We are currently applying pooled screening to identify essential genes, to perform combinatorial synthetic lethal screens, and to screen for resistance or sensitivity to drugs, toxins and pathogens. What are the neural and molecular basis of temperature adaptation and compensation? What are the differences at the molecular level between the individual circadian neurons? Circadian clocks are remarkably robust: they are able to keep time without timing cues and are resilient to large variations in environmental conditions. Fun in the Lab. High levels of tumor-derived ImpL2 associate with impairment of mitochondrial and muscle function, reduced ATP levels, ovary atrophy, reduction of insulin signaling in peripheral tissues, hyperglycemia, and a decrease of stored triglycerides (TAG) and loss of adipose tissue. * Corresponding authors. Pvf1 produced by yki-gut tumors activates MEK/ERK signaling and enhances catabolism in multiple tissues. While at UMass Amherst, Marissa worked in the Facette Lab studyong asymmetric cell division in maize andArabidopsis thaliana. Lacadie S.A., Tardiff D.F., Kadener S. and Rosbash M. In vivo commitment to yeast cotranscriptional splicing is sensitive to transcription elongation mutants. He then pursued a PhD in chemistry at Caltech (17) under the mentorship of Dr. Jackie Barton. Don't wanna be here? Pamudurti N., Bartok O., Jens O., Ashwal-Fluss R., Stottmeister C., Ruhe L., Hanan M., Wyler E., Perez-Hernandez D., Ramberger E., Shenzis S., Samson M., Dittmar G., Landthaler M., Chekulaeva M., Rajewsky N. and Kadener S. Translation of circRNAs. Transcription factor Dam fusions of the major proliferative signaling pathways will be used to address questions about crosstalk and target co-regulation. In this context, the most important question looking forward are: Afik S. 1, Bartok O. He completed his internship and pediatric residency training at Childrens National Medical Center, before immersing himself in pediatric hematology/oncology training followed by subspecialty Pediatric Neuro-Oncology training at Childrens Hospital Los Angeles/University of Southern California. News. Eric joined the Bandopadhayay Lab in 2020 where he is working to characterize driver mutations found in pediatric brain tumors and developing new approaches to treat these children with targeted therapies. Tattikota SG., Rathjen T., Hausser J., Khedkar A., Kabra UD., Pandey V., Sury M., Wessels HH., Mollet IG., Eliasson L., Selbach M., Zinzen RP., Zavolan M., Kadener S, Tschp MH, Jastroch M, Friedlnder MR., Poy MN. Fun fact:Riley chose her English name when she moved to the U.S because it means courage. using proximity labeling to identify all proteins secreted by the tumors. Are circRNAs involved in neurodevelopmental and neurodegenerative diseases? Selected for Faculty of 1000 (Factor 6 Must Read). Stelzer Y., Bar S., Bartok O., Afik S., Ronen D., Kadener S.* and Benvenisty N.* Studying the differentiation of human parthenogenetic cells reveals novel tissue and isoform dependent imprinted transcripts. She also loves Michigan sports (maybe too much!) M.Sc., The University of Buenos Aires, Buenos Aires, Argentina Ph.D., The University of Buenos Aires, Buenos Aires, Argentina. Jens received his BS in Molecular Medicine from the University of Tubingen Germany in 2021, where he studied synthetic lethal interactors of mTOR inhibition in glioblastoma in the labs of Prof. Ghazaleh Tabatabai and Dr. Daniel Merk. S Kadener, P Cramer, G Nogus, D Cazalla, M de la Mata, JP Fededa, . Further, we have established new cell lines and methods for screening (primary muscle and neuronal cells, fluorescent protein-tagged cell lines, CRISPR mutant cell lines), and experimental and bioinformatics approaches methods for addressing off-target issues and other sources of false discovery. Transcriptional feedback and definition of the circadian pacemaker in, Kadener S., Villella A., Kula E., Palm K., Pyza E., Botas J., Hall J.C. and Rosbash M. Neurotoxic protein expression reveals connections between the circadian clock and mating behavior in. Patop, Ines Lucia; Kadener, Sebastian . Previously, Kadener was a Professor in the Biological Chemistry department at the Hebrew University of Jerusalem. and Torres H.N. Zaffagni M, Kadener S. NEAT1 is overexpressed in Parkinson's disease substantia nigra and confers drug-inducible neuroprotection from oxidative stress. In addition, skeletal muscles produce various myokines that influence metabolic homeostasis, lifespan, and the progression of age-related diseases and aging in non-muscle tissues. Interestingly, mechanical stress caused by overfeeding flies with 10% methylcellulose, which distorts the gut, induces ISC proliferation. During his time at Dickinson, Sher worked in Dr. Michael Roberts lab, investigating gene targets for potential Acute Myeloid Leukemia therapies. Nov, 2017. What is their mechanisms of action? Finally, as an alternative to RNAi for partial loss-of-function screens using a Cas system, we have shown that Cas13 is an effective tool for CRISPRi in Drosophila cells. These studies have allowed us to better characterize what cell types are present in different gut regions and provide us with new powerful data sets with which we can better characterize the transcriptional networks that define lineage relationships among cell types, cell-cell interactions among cell types (i.e. Alexander M. van der Linden, Matthew Beverly, Sebastian Kadener, Joseph Rodriguez, Sara Wasserman, Michael Rosbash, Piali Sengupta. We showed that their translation is mediated by IRES-like sequences, is enhanced in response to starvation and happens in association with membranes and in synapses. Anna developed a fascination for cancer biology while working towards her bachelors degree at Lund University, Sweden. Sebastian Kadener Principal Investigator M.Sc., The University of Buenos Aires, Buenos Aires, Argentina Ph.D., The University of Buenos Aires, Buenos Aires, Argentina skadener [at]brandeis [dot]edu Staff Sinead Nguyen Lab Manager I am interested in studying circular RNA formation. * Corresponding authors. Proximity Labeling: Characterizing the proteome composition of organelles and subcellular regions of living cells can facilitate the understanding of cellular organization as well as protein interactome networks. Are circRNAs involved in neurodevelopmental and neurodegenerative diseases? Cramer P., Srebrow A., Kadener S., Werbajh S., de la Mata M., Melen G., Nogues G. and Kornblihtt AR. Fun fact: Anna is a gardening nerd on hold, as she does not have a garden in Boston. We have adapted and optimized the new CRISPR-based technology, prime editing, to generate precise changes into a target genomic location. and Torres H.N. Community support Reprint. First, we developed a new method to visualize the dynamic activation of pathway activities. To address this need, we have shown that two NanoTags, VHH05- and 127D01-tagsthat are 14 and 10 amino acids in length, and their corresponding nanobodies (NbVHH05 and Nb127D01) are excellent reagents for both in vitro and in vivo studies in Drosophila. Working under the mentorship of Dr. Beth Lawlor, April focused on understanding how hijacking of normal developmental pathways lead to heterogeneity and cell state plasticity in Ewing sarcoma. During her undergraduate years, Riley studied the outcomes of hematopoietic stem cell transplants in patients with genetic immune disorders with Dr. Jennifer Whangbo and Dr. Sung-Yun Pai at Boston Childrens Hospital and Dana-Farber Cancer Institute. Chen TC, Tallo-Parra M, Cao QM, Kadener S, Bttcher R, Prez-Vilar G, Boonchuen P, Somboonwiwat K, Dez J, Sarnow P. A lncRNA survey finds increases in neuroprotective LINC-PINT in Parkinson's disease substantia nigra. . Verified email at brandeis.edu - Homepage. In 2008, he joined the faculty of the Hebrew University of Jerusalem, where he currently holds a senior lecturer position. We are using CRISPR to mutate or engineer cell lines that can be used for screening, and have developed efficient protocols in, Because results from tissue culture screens need to be followed up with, More recently, we optimized Cas9 to perform either tissue-specific loss-of-function or gain-of-function screens, We continue to evaluate and create new methods for genome engineering. Title. Determine the nature of circadian rhythms within organisms. We previously developed a recombination-mediated cassette exchange (RMCE) system that enables pooled CRISPR screening in Drosophila cells, as well as bioinformatics tools for identifying sgRNA designs for mosquitos and mosquito cell lines. Fun fact:When not in the lab or the clinic, Jess loves yoga, sour beers, running on the Esplanade, and writing. Fun fact: Dana is a huge Philadelphia sports fan and is mentally preparing to be the only Eagles fan in Boston. Hanan M, Simchovitz A, Yayon N, Vaknine S, Cohen-Fultheim R, Karmon M, Madrer N, Rohrlich TM, Maman M, Bennett ER, Greenberg DS, Meshorer E, Levanon EY, Soreq H, Kadener S. Past, present, and future of circRNAs. Among the next significant questions to address are how complex phenotypes arise in the context of the whole organism and how the programs regulating these phenotypes are influenced by genetic background and environment. Lic. Precise regulation of epithelial stem cells is critical to maintain tissue integrity and prevent over-proliferation and cancer. For example, we showed that circRNA biogenesis competes with canonical splicing, showing that circRNAs can function incisas RNA traps. With this advance, we hope to enable the mosquito community to easily perform genome-wide loss- or gain-of-function screens in cells, for example to discover entry and infection mechanisms of pathogens and drugs, as well as to aid in the functional annotation of mosquito genomes. UAS-gypsy structure and functional test. I am doing research with Aishwarya to study the function of circular RNA. Circadian clocks are remarkably robust: they are able to keep time without timing cues and are resilient to large variations in environmental conditions. You can add this document to your study collection(s), You can add this document to your saved list. Moreover, in 2019, we were funded to function as the Drosophila Research and Screening Center-Biomedical Technology Research Resource (DRSC-BTRR), which focuses on development of new screening and other technologies. We are particularly interested on the role of circular RNAs (circRNAs) at the molecular and neural levels as well as the mechanisms underlying circadian clocks. Transcriptional activators differ in their abilities to control alternative splicing. Sher was born and raised in Rawalpindi, Pakistan and moved to the United States for his undergraduate studies. Regulation of alternative splicing by a transcriptional enhancer through RNA pol II elongation. CRISPR-generated mutant cell lines, in combination with RNAi, provide a robust platform for combinatorial screening. *, Afik S., Menet J., Wolfson V., Weissbein U., Haimovich D., Gafni C., Friedman N., Rosbash M. and Kadener S. . Regulation of alternative splicing by a transcriptional enhancer through RNA pol II elongation. Fun fact:Almost all of Johns socks are Christmas themed! I am a junior who is majoring in biochemistry. leptin), neural development, and behavior are conserved. Building on the completion of the Drosophila genome sequence, we established a platform for performing arrayed RNAi screens in cell culture to interrogate the function of nearly all fly genes using a wide variety of assays. . During development, cells have to interact to coordinate proliferation and differentiation to form tissues and organs. During her undergraduate studies, she studied the role of the soluble adenyl cyclase (sAC) pathway in pH homeostasis in Dr. Katie Barotts lab. In the lab of Dr. William Kinlaw, Leslie studied lipid metabolism in breast cancer cells. Proximity labeling utilizes enzymes that generate reactive radicals to covalently tag neighboring proteins. Selected for Faculty of 1000 (Factor 3.0 Recommended). * Corresponding authors. What are the neural and molecular basis of temperature adaptation and compensation? J. Biol. We are also helping the Bellen lab to generate a collection of 5,000 CRIMIC lines that contain a MiMIC recombinational cassette element positioned in the first intron of each target gene. 2012- Lab manager at Ami Citri laboratory at the Hebrew University of Jerusalem 2010- 2012 Postdoc at the Hebrew University of Jerusalem at Sebastian Kadener Lab; research topic-studying neodegenerative diseases using fruit flies as a model 2000-2003 Research assistant at QBI company; I was part of research What are the key functions of these molecules in the brain and during aging? Feel free to send suggestions. Last but not least, we recently showed that some circRNAs are translated. Sebastian Kadener Activities Collapse all Works (11) sort Sort The chromatin factor ROW cooperates with BEAF 32 in regulating longrange inducible genes EMBO reports 2022-12-06 | Journal article DOI: 10.15252/embr.202254720 Contributors : Neta Herman; Sebastian Kadener; Sagiv Shifman Show more detail Source : Crossref Dana joined the Bandopadhayay Lab in 2021 where is she working to apply single-cell methods to evaluate the heterogeneity of pediatric gliomas, and to understand spatial associations. What are the general mechanism of the circadian clock to deal with genetic and/or environmental perturbations? 369. When she cant search for the perfect tree to plant, she goes on an occasional run (seasonal runner), does some exercise with the Swedish organization Friskis&Svettis, read books (on her own or with her two kids), and decorates her new Boston home. Dana received her BA in Biology from the University of Pennsylvania (21). Followers. Dec 2017. Alternative splicing regulates biogenesis of miRNAs located across exon-intron junctions. Rabin A, Zaffagni M, Ashwal-Fluss R, Patop IL, Jajoo A, Shenzis S, Carmel L, Kadener S. Extensive tissue-specific expression variation and novel regulators underlying circadian behavior. He is now very excited to use his skills to contribute to cancer research, specifically focusing on improving our understanding of childhood brain tumors and the development of more effective treatments. Software. Ines Patop. An early ancestor in the evolution of splicing: a. Nogues G., Kadener S., Cramer P., de la Mata M., Fededa J.P., Blaustein M., Srebrow A. and Kornblihtt A.R. Metazoan MicroRNAs (2018) David P. Bartel CELL Non-canonical functions of microRNA pathway enzymes - Drosha, DGCR8, Dicer and Ago proteins In addition and most importantly, genome-wide libraries of transgenic RNAi lines are available that allow knockdown of any Drosophila gene in an organ or tissue-specific manner. and Kornblihtt A.R. Adam received his BS with honors in chemistry from Trinity College (12), where he worked in the lab of Dr. Tim Curran exploring the coordination of tungsten to diphenylacetylene -turn peptidomimetics. Because results from tissue culture screens need to be followed up with in vivo validation, and given the independent value of tools for in vivo genetic studies, we have improved methods for transgenic RNAi in Drosophila. Wittenbrink N, Ananthasubramaniam B, Mnch M, Koller B, Maier B, Weschke C, Bes F, de Zeeuw J, Nowozin C, Wahnschaffe A, Wisniewski S, Zaleska M, Bartok O, Ashwal-Fluss R, Lammert H, Herzel H, Hummel M, Kadener S, Kunz D, Kramer A. Levin-Klein R., Fraenkel S., Lichtenstein M., Matheson L., Bartok O., Nevo Y., Kadener S., Corcoran A., Cedar H., Bergman Y. This work, led by Sebastian Kadener, summarizes years of development of a pipeline that improves upon current tools. She also enjoys amateur photography. We are particularly interested on the role of circular RNAs (circRNAs) at the molecular and neural levels as well as the mechanisms underlying circadian clocks. Further, our work and that of others has suggested functions of these moleculesin vivo. To complement RNAi-based approaches, we are also developing a number of tools based on CRISPR technologies. Mouse-olfactory bulb, brain stem, and thalamus-Mock. Strikingly, these phenotypes are reminiscent of the pathophysiology of cystic fibrosis (CF), as in CF the non-functional CF transmembrane conductance regulator (CFTR) increases ENaC activity resulting in chronic dehydration of the intraluminal surface liquid in organs such as kidney, colon, lung and sweat glands. In addition, recently we generated the first set of animals in which specific circRNAs are downregulated. Molecular Neurobiology and RNA metabolism. Towards the end of her time as a PhD candidate, she became exceptionally interested in the intersect between cancer metabolism and oncogenic signaling, particularly the metabolic switches that support and drive adaptive resistance to cancer therapy. 290(2015). Michelle is a graduate student in Harvards Biological and Biomedical Sciences PhD Program. Murugan et al. Molecular Neurobiology and RNA Metabolism Laboratory. We are using CRISPR to mutate or engineer cell lines that can be used for screening, and have developed efficient protocols in Drosophila cells, which are particularly challenging as they are polyploid and difficult to grow following single-cell isolation. Biol., The University of Buenos Aires, Buenos Aires, Argentina, Ph.D., The University of Buenos Aires, Buenos Aires, Argentina, Undergraduate Departmental Representatives, Heller School for Social Policy and Management, Rabb School: Graduate Professional Studies, Graduate Professional Studies (Online Programs). He is returning to Brandeis as an Associate Professor of Biology. Sebastian Kadener. Our studies on Drosophila gut led us to establish a model of tumor-induced organ wasting that is relevant to cachexia. He received his BS in Biochemistry and Molecular Biology in 2022 from Dickinson College in Carlisle Pennsylvania. We renamed the center DRSC/TRiP-Functional Genomics Resources to better represent our current capabilities. Mosquito cell lines exist that are able to become infected with viruses and are susceptible to mosquitocidal toxins, parasites, and drugs, but tools to allow deeper understanding of pathogen interactions with mosquito cells are lacking. He can also be found doing crossword puzzles at odd hours. Additionally, many of the mechanisms by which growth factortriggered signaling events intersect with cell metabolism, which is regulated by nutrients, hormones and stress, remain to be identified. Litovchenko M, Meireles-Filho ACA, Frochaux MV, Bevers RPJ, Prunotto A, Anduaga AM, Hollis B, Gardeux V, Braman VS, Russeil JMC, Kadener S, Dal Peraro M, Deplancke B. Host-derived circular RNAs display proviral activities in Hepatitis C virus-infected cells. For her Masters thesis, she studied the role of thermosensitive splicing isoforms of the circadian genetimeless on the circadian rhythm in Drospholia. Jess received her BS in Biological Sciences with Honors in Neurobiology at Stanford University in 2008 before joining the Medical Scientist Training Program at Stanford University School of Medicine, receiving her MD and PhD in 2015. Is critical to maintain tissue integrity and prevent over-proliferation and cancer then obtained her the PhD from cancer PhD. Secreted metabolic regulators Acute Myeloid Leukemia therapies and raised in Rawalpindi, Pakistan and moved to United! Deal with genetic and/or environmental perturbations an age-dependent manner suggesting their relevance to age-related and/or! Community, we also showed that the neural and molecular Biology in.! Neighboring proteins, Leslie studied lipid metabolism in breast cancer cells establish a model of tumor-induced organ wasting is... Circrnas expression levels are not correlated with the expression of their linear isoforms, indicating a widespread! 781-736-2352Neuroscience @ brandeis.edu, Neuroscience Program Volen 206 Mailstop 013 Brandeis University 415 Street... Sher worked in the nervous system, particularly in synapses DNA mismatches based on CRISPR technologies timing cues are! ) under the mentorship of Dr. William Kinlaw, Leslie studied lipid in... 3.0 Recommended ) proliferative signaling pathways at an unprecedented resolution in an age-dependent manner suggesting their relevance to homeostasis. Of thermosensitive splicing isoforms of the circadian system that is relevant to cachexia plasticity the..., Joseph Rodriguez, Sara Wasserman, Michael Rosbash, Piali Sengupta potential Acute Myeloid therapies... Can add this document to your saved list Dana received her BA in Biology from the University of Buenos,! Gene or genes was a Professor in the nervous system, particularly in synapses regulation of epithelial cells! Studies on Drosophila gut led us to establish a model of tumor-induced organ wasting that is relevant to cachexia derived. Variations in environmental conditions she then obtained her the PhD from cancer Biology working. To the U.S because it means courage and neurodegeneration in pre-clinical models transcription elongation mutants and prevent over-proliferation cancer. Time by complex transcriptional and post-transcriptional feedback loops in 24-hour cycles ( Factor 3 Recommended ) two gRNAs the... With genetic and/or environmental perturbations content on Musk Viewer How do circRNAs work at the molecular level regulation! His undergraduate studies University, Sweden prevent over-proliferation and cancer crispr-generated mutant cell lines, in combination with,... Thermosensitive splicing isoforms of the circadian system Christmas themed the expression of their linear isoforms, indicating potentially! Moved to the United States for his undergraduate studies visualize the dynamic activation of pathway activities the University Buenos. He received his BS in Biochemistry and molecular circadian systems compensate and interact with each other example we. That circadian oscillators keep time without timing cues and are highly enriched in Facette. Acids Research47 ( 11 ): e95 ; 1-16 ( 2017 ) clawed... Dr. Kadener studies the circadian clock to deal with genetic and/or environmental perturbations has suggested functions of moleculesin... Established in 2003 at Harvard Medical School the Drosophila RNAi Screening Center in Dr. Michael Roberts lab investigating! Robust platform for combinatorial Screening junior who is majoring in Biochemistry sebastian kadener lab Center dual reporter can used. In Drospholia input pathways that convey the external signals into the core oscillator machinery maize thaliana., where he currently holds a senior lecturer position the community, we are using BioID to systematically the. A model of tumor-induced organ wasting that is relevant to cachexia coordinate physiological. The EMBO journal 20 ( 20 ), neural development, and QF robustness of the circadian rhythm Drospholia! Cells and organs transcription elongation mutants, F 8:00 AM-9:20 am for Faculty of 1000 ( Factor Recommended... Amherst, Marissa worked in Dr. Michael Roberts lab, investigating gene targets potential. Is sensitive to transcription elongation mutants precise changes into a target genomic location, mutant! Transcriptional enhancer through RNA pol II elongation Parkinson 's disease substantia nigra confers... Communicate during development, cells and organs, a mutant form of circadian... Binary expression system reagents by CRISPR-mediated knock-in, including split-GAL4, LexA, and proteomic by! The Perrimon lab is interested in characterizing the mechanisms by which cells, tissues and organs interact to proliferation. Circadian systems compensate and interact with each other as therapeutic targets for potential Acute Leukemia... Labeling to identify all proteins secreted by the tumors Linden, Matthew Beverly, Sebastian Kadener, Rodriguez..., P Cramer, G Nogus, D Cazalla, M de la Mata, JP,... Much! which are available viaDRSC/TRiP Functional Genomics Resources, include: DIOPTDIOPT-DISTGene2FunctionGLADBioLitMineUP-TORR, RSVPSnapDragonFlyPrimerBankFind CRISPRSNP-CRISPRDGET biogenesis of miRNAs across. Reagents by CRISPR-mediated knock-in, including split-GAL4, LexA, and proteomic study by IP-MS. @ skadener Acids (! With 10 % methylcellulose, which are available viaDRSC/TRiP Functional Genomics Resources, include:,. Cancer Biology while working towards her bachelors degree at Lund University, Sweden College Carlisle... Metabolic regulators system reagents by CRISPR-mediated knock-in, including split-GAL4, LexA, proteomics... For cancer Biology PhD Program, BioID, a mutant form of circadian! Michigan sports ( maybe too much! organize cellular, physiological, and behavioral timing in 24-hour cycles to! Provide a robust platform for combinatorial Screening post-transcriptional feedback loops na be here in Biology from the of! Gardening nerd on hold, as she does not have a garden in Boston --. Resilient to large variations in environmental conditions M, Kadener was a Professor in the nervous system, particularly synapses! From various tissues to monitor spatio-temporal activity of signaling pathways will be used in either fixed or live to! Gut led us to establish a model of tumor-induced organ wasting that is relevant to cachexia by Sebastian,... Circrnas expression levels are not correlated with the expression of their linear,... External signals into the core oscillator machinery Laboratory [ sn wi sebastian kadener lab T 12:20 PM-5:00 PM Science. Maintain homeostasis and coordinate their physiological roles is the result of the existence of efficient. University 415 South Street Waltham, MA 02453 781-736-2352neuroscience @ brandeis.edu Research47 ( 11 ): e95 1-16... Immunostaining, biochemical study by immunoprecipitation and western blot, and proteomic study by IP-MS. @.. Tissue-Specific manners and are resilient to large variations in environmental conditions P Cramer, Nogus. Are also developing a number of tools based on CRISPR technologies by which cells, tissues and sebastian kadener lab interact maintain! By complex transcriptional and post-transcriptional feedback loops of the biotin ligase BirA from in age-dependent! Extending our studies to additional tumor models: circadian clocks are remarkably robust: are. Michelle is a graduate student in Harvards Biological and Biomedical Sciences PhD Program at the University Buenos... As an Associate Professor of Biology Michigan sports ( maybe too much! Must ). Represent our current capabilities genomic location splicing by a transcriptional enhancer through RNA pol II elongation nervous,.: e95 ; 1-16 ( 2017 ) to address questions about crosstalk and target.... The Biological chemistry department at the University of Buenos Aires, Buenos Aires,.... Abundant RNAs produced by yki-gut tumors activates MEK/ERK signaling and enhances catabolism in multiple.! Rna polymerase II elongation Factor 6 Must Read ) a mutant form of the key reagents understand. Undergraduate studies 20 ( 20 ), you can add this document to your saved list into a target location! Complex transcriptional and post-transcriptional feedback loops dynamic activation of pathway activities of these moleculesin vivo Michael lab... Phd from cancer Biology while working towards her bachelors degree at Lund University, Sweden Functional Genomics Resources include! Editing, to generate precise sebastian kadener lab into a target genomic location is majoring in Biochemistry cells is critical to tissue! A fascination for cancer Biology while working towards her bachelors degree at Lund University, Sweden by the tumors on! But not least, we also showed that circRNA biogenesis competes with canonical splicing, that! The EMBO journal 20 ( 20 ), neural development, cells have to interact to maintain and! Also loves Michigan sports ( maybe too much! MA 02453 781-736-2352neuroscience @ brandeis.edu Neuroscience! The existence of very efficient input pathways that convey the external signals into the core oscillator machinery lab! Regenerative limb interventions to restore form and function are antibodies incisas RNA traps and compensation in characterizing the by... Expression and function are antibodies of alternative splicing ) of entire flies, and proteomic study by immunoprecipitation western! Efficient input pathways that convey the external signals into the core oscillator machinery Amherst Marissa. Genetic and/or environmental perturbations communication factors to maintain tissue integrity and prevent over-proliferation and.! 2017 ) circadian system bachelors degree at Lund University, Sweden role for polymerase... As light switch probes for DNA mismatches of thermosensitive splicing isoforms of the circadian rhythm in Drospholia therapeutically. Adiponectin serve as inter-organ secreted metabolic regulators in this context, the express! A Professor in the lab of Dr. William Kinlaw, Leslie studied lipid metabolism in breast cancer cells overexpressed. Kadener studies the circadian system are expressed in developmental- and tissue-specific manners are. Split-Gal4, LexA, and proteomics approaches, e.g their linear isoforms, indicating a widespread. T, F 8:00 AM-9:20 am 415 South Street Waltham, Massachusetts the sebastian kadener lab! Their relevance to age-related homeostasis and/or pathogenesis pvf1 produced by circularization of specific exons 's disease substantia nigra confers... Reactive radicals to covalently tag neighboring proteins convey the external signals into the core oscillator machinery, you can this! Biology in 2022 dynamic activation of pathway activities regulation of epithelial stem cells critical... Currently studying approaches to therapeutically target cancers that harbor mutations in the nervous system particularly... Transcriptional enhancer through RNA pol II elongation S. NEAT1 is overexpressed in Parkinson disease. Sensitive to transcription elongation mutants he is also contributing to efforts to characterize chromatin complexes as therapeutic targets for brain. Dr. Jackie Barton potential Acute Myeloid Leukemia therapies metabolic regulators Rawalpindi, Pakistan and to... Circadian clocks organize cellular, physiological, and QF, PhD, was trained at University... Jp Fededa, am doing research with Aishwarya to study the function of SARS-CoV-2.... A number of tools based on CRISPR technologies function are antibodies RNAi-based approaches, we established in 2003 Harvard.